ftsE [2018-02-10T10:58:15.000Z]

[SW|ABC transporter ](ATP-binding protein) ([protein|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|FtsE]-[protein|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|FtsX]), required for [protein|search|CwlO ]activity (cell elongation) and for proper activation of [protein|search|Spo0A ]and initiation of [SW|sporulation]

locus: BSU35260

pI: 8.42

mw: 25.47 kDa

protein length: 228 aa

gene length: 684 bp

function: control of [SW|cell wall synthesis]

product: [SW|ABC transporter ](ATP-binding protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.2|Autolytic activity required for peptidoglycan synthesis (cell elongation)]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.3|Regulatory ABC transporters]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,624,821 → 3,625,507

Phenotypes of a mutant

• a ''[gene|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|ftsE]'' mutation is synthetically lethal with a ''[gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE]'' mutation (due to a lack of autolysin activity) [Pubmed|23869552,23855774]
• shorter, fatter cells, this can be rescued by addition of Mg(2+) [Pubmed|23869552,23855774]
• reduced growth rate, especially at low Mg(2+) concentrations [Pubmed|23869552]

The protein

Catalyzed reaction/ biological activity

• necessary for the autolysin activity of [protein|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|CwlO] [Pubmed|23855774]

Protein family

• [SW|ABC transporter] family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|YknY], [protein|4FE6A51A0D9BBBF574EA2EF18B95B5BFC0A64CCB|YtrE]

Structure

• [PDB|3TIF] (ATP-binding protein from ''Methanococcus jannaschii'', 40% identity) [Pubmed|22158619]

[SW|Localization]

• membrane associated (via [protein|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|FtsX]) [Pubmed|18573177]

Expression and Regulation

Operons

• genes: [gene|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|ftsE]-[gene|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|ftsX]
  description: (according to [http://dbtbs.hgc.jp/COG/prom/ftsEX.html DBTBS]) null

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Biological materials

Mutant

• BKE35260 (Δ[gene|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|ftsE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAATCACCTAATCTTT, downstream forward: _UP4_GAGTCAAGAGGGGAGTATGG
• BKK35260 (Δ[gene|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|ftsE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAATCACCTAATCTTT, downstream forward: _UP4_GAGTCAAGAGGGGAGTATGG

References

• 10092453,18573177,22158619,14651647,23855774,23869552